Heterotopic transplantation of a decellularized and recellularized whole porcine heart

57 views Leave a comment

Decellularization of a whole porcine heart. (A–E) Representative perceivable images of a porcine heart during a decellularization routine during 0, 3, 6, 9 and 12 h. (F) Representative sketch of a perfusion–decellularization complement (1: drum pump, 2: feverishness exchanger, 3: vigour monitor, 4: heart chamber). (G–J) Haematoxylin and eosin dirty of a normal heart (G) and decellularized heart (I). 4′,6-Diamidino-2-phynylindole dirty of a normal heart (H) and a decellularized heart (J). Scale bar: 200 μm.

OBJECTIVES:

One of a final treatments for end-stage heart disaster is heart transplantation. However, a necessity of donor hearts has combined a prolonged watchful list and singular benefits. Our ultimate idea is to emanate a whole violence heart built on an organ skeleton for tellurian heart transplantation. Here, we successfully achieved a initial transplantation regulating a decellularized whole porcine heart with mesenchymal branch cells.

METHODS:

A porcine heart was harvested following cardiac detain prompted by a high-potassium resolution and stored during -80°C for 24 h. The porcine heart was totally decellularized with 1% sodium dodecyl sulphate and 1% Triton X-100 underneath a control of perfusion vigour (100 mmHg) and confirmed during 37°C. A decellularized whole-heart skeleton was sterilized with gamma irradiation. Cultured mesenchymal branch cells were collected and possibly infused into a descending aorta or injected directly into a left ventricular wall. Finally, recellularized whole-heart scaffolds were transplanted into pigs underneath systemic anticoagulation diagnosis with heparin. Coronary artery angiography of a transplanted heart swindle was performed.

RESULTS:

In a decellularization method, all mobile components were removed, preserving a heart extracellular matrix. Heterotopic transplantations were successfully achieved regulating a decellularized heart and a recellularized heart. The scaffolds were good perfused, but draining from a aspect or anastomosis site. Coronary angiography suggested a obvious coronary artery in both scaffolds. The transplanted decellularized heart was harvested on Day 3. Haematoxylin and eosin dirty showed thrombosis in a coronary arteries and migrated inflammatorycells. Haematoxylin and eosin dirty of a transplanted recellularized heart showed identical findings, with a difference of injected mesenchymal branch cells.

CONCLUSIONS:

To a best of a knowledge, this is a initial news of heterotopic transplantation of a decellularized whole porcine heart withmesenchymal branch cells. The scaffolds endured surgical procedures. We rescued short-term coronary artery perfusion in a transplanted scaffolds by angiography. Future studies should analyse a histological facilities of transplanted decellularized scaffolds and optimize a complement for recellularization to request this singular record clinically.

Source: PubMed