Optimal timing of mesenchymal branch cells therapy for neonatal intraventricular hemorrhage

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OBJECTIVE:

We recently showed that intraventricular transplantation of tellurian umbilical cord blood (UCB)-derived mesenchymal branch cells (MSC) significantly dragging posthemorrhagic hydrocephalus (PHH) and mind damage after serious intraventricular hemorrhage (IVH) in baby rodent pups. The purpose of this investigate was to optimize a timing of MSC transplantation for serious IVH.

METHODS:

Severe IVH was prompted by injecting 100 uL of blood into any ventricle of Sprague-Dawley rats on postnatal day 4 (P4). Human umbilical cord blood (UCB)-derived MSCs (1×105 cells in 10ul of normal saline) were transplanted intraventricularly underneath stereotaxic superintendence possibly early during P6 or late during P11. Serial mind MRIs and behavioral duty tests, such as disastrous geotaxis and rotarod tests, were performed. At P32, mind hankie samples were performed for histological and biochemical analyses.

RESULTS:

Intracerebroventricular transplantation of MSCs significantly dragging a growth of PHH, decreased marred behavioral tests, increasing TUNEL-positive cells, increasing astrogliosis, reduced corpus callosum thickness, reduced myelin simple protein expression, and increasing a inflammatory cytokines interleukin (IL)-1α, IL-1β, IL-6, and growth necrosis factor-α during P6 though not during P11 after initiation of serious IVH.

CONCLUSIONS:

Intracerebroventricular transplantation of tellurian UCB-derived MSCs dragging PHH and mind damage after serious IVH in baby rats in a time-dependent manner. Significant neuroprotection was usually demonstrated when administered early during 2 days after initiation though not late during 7 days after initiation of serious IVH.

Source: PubMed